Clinical Trial: Changes in Alveolar Macrophage Function During Acute Lung Injury

Study Status: Not yet recruiting
Recruit Status: Not yet recruiting
Study Type: Observational

Official Title: Changes in Alveolar Macrophage Function During Acute Lung Injury

Brief Summary: In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.

Detailed Summary: Alveolar macrophages are tissue-resident or recruited cells with key functions in recognition of pathogens, initiation of host defensive ang protective inflammation,and in clearance of pathogens from the airways. However, macrophages are also responsible for efficient clearance of apoptotic neutrophils, termed efferocytosis, which is a prerequisite for the timely resolution of inflammation. In this study, the investigators aim to observe the change in alveolar macrophage function during acute lung injury.
Sponsor: West China Hospital

Current Primary Outcome: alveolar macrophages (AMs) phagocytic index [ Time Frame: 7 days after admission ]

AMs will be collected and cultured in vitro. Apoptotic neutrophils will then be cocultured with AMs for 1 hour. To measure phagocytosis of apoptotic neutrophils, AMs were further processed and phagocytosed apoptotic neutrophils were detected by confocal fluorescence microscopy as described below under Cell staining and confocal microscopy. The phagocytic index was calculated according to the following formula: phagocytic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.


Original Primary Outcome: AM phagocytic index [ Time Frame: 7 days after admission ]

AM will be collected and cultured in vitro. Apoptotic neutrophils will then be cocultured with AMs for 1 hour. To measure phagocytosis of apoptotic neutrophils, AM were further processed and phagocytosed apoptotic neutrophils were detected by confocal fluorescence microscopy as described below under Cell staining and confocal microscopy. The phagocytic index was calculated according to the following formula: phagocytic index = (total number of engulfed cells/total number of counted macrophages) × (number of macrophages containing engulfed cells/total number of counted macrophages) × 100.


Current Secondary Outcome: 30 days mortality [ Time Frame: 30 days after admission ]

Original Secondary Outcome: Same as current

Information By: West China Hospital

Dates:
Date Received: February 11, 2016
Date Started: September 2016
Date Completion: August 2018
Last Updated: February 26, 2016
Last Verified: February 2016