Clinical Trial: Spermatogonial Stem Cells in Azoospermic Patients: a Comparison Between Obstructive and Non-obstructive Azoospermia

Study Status: Recruiting
Recruit Status: Unknown status
Study Type: Observational

Official Title:

Brief Summary: The researchers hypothesized that nonobstructive azoospermia might be associated with a reduction OF SSCs in seminiferous tubules as compared with obstructive azoospermia. Testicular sperm specimens that had been previously extracted from azoospermic patients will be paraffin embedded and stained with anti GPR-125. After antigen retrieval, the sections will be incubated with rabbit anti GPR125 followed by Cy3-conjugated anti rabbit secondary antibodies. Those found to be SSCs will be counted using light microscopy, and compared between patients with obstructive (n=11) and non-obstructive azoospermia (n=9).

Detailed Summary:
Sponsor: Hillel Yaffe Medical Center

Current Primary Outcome: SSCs were identified in both obstructive and nonobstructive azoospermia patients. [ Time Frame: One year ]

Original Primary Outcome: Same as current

Current Secondary Outcome:

Original Secondary Outcome:

Information By: Hillel Yaffe Medical Center

Dates:
Date Received: August 9, 2010
Date Started: January 2010
Date Completion: October 2010
Last Updated: August 9, 2010
Last Verified: August 2010