Clinical Trial: Research for Specific Proteins of Interest for the Serological Diagnosis of Bird Fancier's Lung

Study Status: Recruiting
Recruit Status: Recruiting
Study Type: Interventional

Official Title: Research for Specific Proteins of Interest for the Serological Diagnosis of Bird Fancier's Lung

Brief Summary: This study aims at identifying bird proteins useful for diagnostic tests to determine the cause of Bird Fancier's Lung (BFL).

Detailed Summary:

Allergic diseases are an important part of health concerns in Europe and in countries "industrialized". Among them are distinguished from hypersensitivity pneumonitis (HP), consecutive to repeated inhalation of organic substances (bacteria, fungi, avian proteins ...).

As with other HP, the BFL is a invalidating disease, but usually not lethal if the patient reduced sufficiently early exposure to antigens.

Associated symptoms include breathing difficulties a dry cough, a general fatigued state accompanied by a fever and a rapid and pronounced weight loss.

These clinical signs may be similar to a flu-like condition, difficult to discern from other respiratory disease. Diagnosis is based on a set of clinical, radiological and biological arguments that are often discussed.

Using these criteria as predictors would increase opportunities to diagnose HP without using invasive clinical examinations such as biopsy and bronchoalveolar lavage.

Although serology is a useful argument for the diagnosis, its value is often criticized.

Thus, significant variations in performance of serological tests are observed from one provider to another and, for the same supplier, from one batch to another.

This issue could be raised by the use of recombinant antigens. The principle is to use only to the reaction the purified protein of interest produced by genetic engineering.

The Parasitology-Mycology department has acquired expertise in the research of proteins of interest for serology allergic diseases and in the manufacture of recombinant ant
Sponsor: Centre Hospitalier Universitaire de Besancon

Current Primary Outcome: number of protein of interest [ Time Frame: 24 months ]

5 and 15 proteins for all selected birds


Original Primary Outcome: Same as current

Current Secondary Outcome:

  • Selection and identification of protein of interest specific for each selected bird [ Time Frame: 24 months ]
    Identifying at least three specific proteins for each bird species by 2D-Electrophoresis and Western blotting (based on previous study).
  • Selection and identification of proteins of interest common to all selected bird'droppings [ Time Frame: 24 months ]
    Identifying at least one common protein to all droppings of selected bird species by 2D-Electrophoresis and Western blotting. This protein will be recognized by the antibodies of all patients regardless of their exposure and not recognized by controls. It would make the diagnosis of BFL for any avian exposure.


Original Secondary Outcome: Same as current

Information By: Centre Hospitalier Universitaire de Besancon

Dates:
Date Received: August 10, 2016
Date Started: January 2016
Date Completion: December 2018
Last Updated: February 14, 2017
Last Verified: August 2016