Clinical Trial: Study of Synapsis and Recombination in Male Meiosis and the Implications in Infertility

Study Status: Completed
Recruit Status: Completed
Study Type: Observational

Official Title: Study of Synapsis and Recombination in Male Meiosis and the Implications in Infertility

Brief Summary: The aim of this study was to assess meiotic recombination in primary spermatocytes, synaptonemal complex length and the correlation with chromosomal abnormalities in testicular spermatozoa from infertile men with idiopathic non-obstructive azoospermia (NOA).

Detailed Summary: During the first meiotic division in spermatogenesis there are two critical events. First, synapsis between homologous chromosomes and formation of the synaptonemal complex (SC), which regulates sister chromatid cohesion and provides the template for localization of recombination machinery proteins. Secondly, recombination between homologous chromosomes, which is essential for the correct segregation. Errors during these two processes may induce incorrect segregation of chromosomes and are a major cause of gamete aneuploidy. The aim of this study was to assess meiotic recombination in primary spermatocytes, synaptonemal complex length and the correlation with chromosomal abnormalities in testicular spermatozoa from infertile men with idiopathic non-obstructive azoospermia (NOA). Prospective cohort study to assess meiotic progression, total length of SC, frequency of recombination and sperm aneuploidy in samples obtained from testicular biopsies from NOA patients. The study group was compared with a control group from post-vasectomized (OA) patients. Immunocytogenetics with SCYP3, CREST and MLH1 antibodies for meiotic progression, SC length, and recombination. Fluorescence in situ Hybridization (FISH) for chromosomes 1, 4, 6, 13, 16, 18, 21, 22 in primary spermatocytes and chromosomes 13, 18, 21, X, Y on sperm. MicroMeasure 3.3 program was used for SC length.
Sponsor: Igenomix

Current Primary Outcome: Meiotic recombination [ Time Frame: once ]

Number of MLH1 foci in pachytene cells


Original Primary Outcome: Same as current

Current Secondary Outcome: Sperm aneuploidy [ Time Frame: once ]

Percentage of sperm with aneuploidies


Original Secondary Outcome: Same as current

Information By: Igenomix

Dates:
Date Received: January 12, 2015
Date Started: January 2008
Date Completion:
Last Updated: January 14, 2015
Last Verified: January 2015