Clinical Trial: DNA Analysis of Tissue From Patients With T-Cell Acute Lymphoblastic Leukemia
Study Status: Completed
Recruit Status: Completed
Study Type: Observational
Official Title: Molecular Mechanisms of NOTCH Induced Transformation in T-ALL
Brief Summary:
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.
PURPOSE: This laboratory study is looking at tissue samples from patients with T-cell acute lymphoblastic leukemia.
Detailed Summary:
OBJECTIVES:
- Identify the transcriptional signatures associated with the presence of NOTCH1 mutations in primary T-cell acute lymphoblastic leukemia (T-ALL) cells using oligonucleotide microarrays.
- Characterize the global changes on gene expression resulting from the inactivation of NOTCH signaling in human T-ALL lymphoblasts.
OUTLINE: This is a multicenter study.
Frozen lymphoblast samples from patients with T-cell acute lymphoblastic leukemia (NOTCH1-mutated and wild type) are assessed for genetic expression profiles and mutations by microarray analysis and activated NOTCH1 protein by western blot analysis.
PROJECTED ACCRUAL: A total of 48 samples from patients will be accrued for this study.
Sponsor: ECOG-ACRIN Cancer Research Group
Current Primary Outcome:
- Transcriptional signatures associated with NOTCH1 mutations in T-cell acute lymphoblastic leukemia (T-ALL) cells [ Time Frame: 1 month ]
- Global changes on gene expression resulting from inactivation of NOTCH signaling in T-ALL cells [ Time Frame: 1 month ]
Original Primary Outcome:
- Transcriptional signatures associated with NOTCH1 mutations in T-cell acute lymphoblastic leukemia (T-ALL) cells
- Global changes on gene expression resulting from inactivation of NOTCH signaling in T-ALL cells
Current Secondary Outcome:
Original Secondary Outcome:
Information By: Eastern Cooperative Oncology Group
Dates:
Date Received: May 9, 2009
Date Started: November 30, 2006
Date Completion:
Last Updated: May 17, 2017
Last Verified: May 2017