Clinical Trial: DNA Analysis of Tissue From Patients With T-Cell Acute Lymphoblastic Leukemia

Study Status: Completed
Recruit Status: Completed
Study Type: Observational

Official Title: Molecular Mechanisms of NOTCH Induced Transformation in T-ALL

Brief Summary:

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer.

PURPOSE: This laboratory study is looking at tissue samples from patients with T-cell acute lymphoblastic leukemia.


Detailed Summary:

OBJECTIVES:

  • Identify the transcriptional signatures associated with the presence of NOTCH1 mutations in primary T-cell acute lymphoblastic leukemia (T-ALL) cells using oligonucleotide microarrays.
  • Characterize the global changes on gene expression resulting from the inactivation of NOTCH signaling in human T-ALL lymphoblasts.

OUTLINE: This is a multicenter study.

Frozen lymphoblast samples from patients with T-cell acute lymphoblastic leukemia (NOTCH1-mutated and wild type) are assessed for genetic expression profiles and mutations by microarray analysis and activated NOTCH1 protein by western blot analysis.

PROJECTED ACCRUAL: A total of 48 samples from patients will be accrued for this study.


Sponsor: ECOG-ACRIN Cancer Research Group

Current Primary Outcome:

  • Transcriptional signatures associated with NOTCH1 mutations in T-cell acute lymphoblastic leukemia (T-ALL) cells [ Time Frame: 1 month ]
  • Global changes on gene expression resulting from inactivation of NOTCH signaling in T-ALL cells [ Time Frame: 1 month ]


Original Primary Outcome:

  • Transcriptional signatures associated with NOTCH1 mutations in T-cell acute lymphoblastic leukemia (T-ALL) cells
  • Global changes on gene expression resulting from inactivation of NOTCH signaling in T-ALL cells


Current Secondary Outcome:

Original Secondary Outcome:

Information By: Eastern Cooperative Oncology Group

Dates:
Date Received: May 9, 2009
Date Started: November 30, 2006
Date Completion:
Last Updated: May 17, 2017
Last Verified: May 2017